[Documentation] DNA measurement

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(Robert Giessmann) #1

DIY Fluorometer

Checking quantity of DNA, reducing waste and time.

We also have a documentation on github: https://diy-fluorometer.github.io/documentation/

TEAM

  • Michal Koudelka @gen-failure [OTS discourse: @Michal_Koudelka ]
  • Thomas Mboa @thomasmboa [OTS discourse: @thomasmboa ]
  • Martin , @xMartin [OTS discourse: @xMartin ]
  • Patrick @p---
  • Artiom Magomedov email him here! [OTS discourse: @Artiom ]
  • Moritz Ebeling-Rump @gen-failure
  • Lisa @lthalheimer [OTS discourse: @lisa ]
  • Robert @rgiessmann [OTS discourse: @r.giessmann ]
  • Theron @pftburger [OTS discourse: @pftburger ]
  • MATERIALS

    • for the case:
      • MDF wood (3mm)
      • aluminum foil
      • lots of tape
    • for the measurement device / electronics:
    • for the server / client device; display:
      • Raspberry Pi
    • for the experiments:
      • Biozym 10 uL SafeSeal-Tips filter low binding
      • DNA dye: GelGreen
      • DNA sample: BIORON 1kb DNA Ladder (no stain), 0.2 mg/mL
      • Eppendorf micropipettors
      • 0.2 mL PCR tubes

    tools:

    • laser cutter
    • hackers and their brains!

    HOW WE BUILT IT

    please check the general project documentation on

    PHOTOS

    CODE

    check on github:

    LINKS

    As you might have guessed already:

    NEXT ITERATIONS

    • improving the optics (filters, LED)
    • integrating the electronics into the case
    • making the server reachable from other devices, e.g. smartphones, laptops, …

    (Lucy Patterson) #2

    Hi Robert! It’d be great if you could edit the post to include the OTS usernames of everyone in the team list. That way the whole team will get notifications if someone comments on this thread. (As you can see, I tried to do it for you, but seems it’s only the original author who has the rights to do this.) There is someone who wants to speak to you… Thanks!


    (Robert Giessmann) #3

    ping to OTS users: @Michal_Koudelka @thomasmboa @xMartin @Artiom @lisa @r.giessmann @pftburger


    (Martin Stadler) #4

    I uploaded some more photos: https://github.com/diy-fluorometer/documentation/tree/master/fotos


    (Lucy Patterson) #5

    Hey! I just heard about this DIY fluorometer project from a EU grant a couple of years ago… https://github.com/BioDesignRealWorld/FluoMeter
    …just in case it’s useful. Did you guys know about it?


    (Detlef Niese) #6

    Hi! Great stuff. Would love bein able to use it with my students but have no access to a laser cutter. Any standard sketch availabe with necesary measures so I can buld the housing manually? Thanks